Mass spectrometry

Objectives & applications

The INCI Mass Spectrometry technical platform aims to respond to the variety of analysis requests and to offer solutions adapted to the identification and quantification of molecules of interest in various fields of biology and of biochemistry.

Applications: small peptide or non-peptide molecules, metabolomics.


Support, extraction and analysis services using a latest generation triple quadrupole mass spectrometer (MS) (Endura, ThermoFisher Scientific) (2014) (2014) are offered. This equipment allows the quantitative analysis of molecules with a mass range of 10 to 3400 m/z. Two ionization sources, electrospray (HESI) and atmospheric pressure chemical ionization (APCI), are available depending on the problems encountered.

This mass spectrometer can either be used in direct infusion, or coupled with a Dionex Ultimate 3000 UPLC chain (ThermoFisher Scientific) which allows rapid analyzes of complex samples. Different analysis modes are available: full scan, precursor ion scan, product ion scan, loss of neutral and SRM scan.

On the other hand, this device offers high sensitivity combined with low noise for the quantitative analysis of small molecules, peptides, biosimilar and biological substances. More signal and less noise translate into greater dosing precision and accuracy.

Specification of ENDURA
  • Commercialized device acquired in March 2014.
  • Mass range 10-3400 m / z.
  • Presence of a neutral ion blocker guide (avoids clogging and increases sensitivity).
  • Hyperbolic quadrupole allowing to work at high resolution without loss of sensitivity.
  • Active collision cell (improves ion fragmentation and transit speed) 15000 amu / s, 500srm / s without loss of sensitivity.
  • Precision 0.4 in high resolution.
  • Detector for working in a wide dynamic range (106) and particularly when the ion flow is low (“dual mode discrete dynode detector,” counting mode and analog mode).
  • Failover polarity <20ms.

Scientific Director : Dr. Yannick Goumon, CRCN Inserm

Technical director : Virginie Andry, IE CNRS

Phone : 03 88 45 67 18

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