Behavioral experiment plateform
In coordination with the Chronobiotron UMS3415, this technical platform focuses on rodents (rats and mice) behavior in particular in models and tests of pain and anxio-depressive disorders.
We combine genetic approaches (transgenic mice, siRNA, viral transgenesis, optogenetic, chemogenetic …) with molecular and physiological measurements to understand the biological bases of behaviors studie.
Mélanie KREMER
Director,
technical and administrative manager
Ipek Yalcin
Scientific manager
Victor MATHIS
cdd researcher
Noémie WILLEM
Study engineer
Aurélia CES
Assistant engineer
Our skills
- Neuropathic pain: injury models (“cuff”, “chronic constriction injury”, “spared nerve injury”), genetic model (diabetic neuropathy: ob/ob mice), chemo-induced models.
- Inflammatory pain: carrageenan, formalin, complete Freund's adjuvant
- Opiate-induced hyperalgesia, tolerance, withdrawal
- tail flick
- tail immersion
- hot plate
- cold plate
- Hargreaves Plantar®
- thermal place preference
Mechanical:
- Von Frey (manuel et électronique)
- tail pressure test
- calibrated forceps
Spontaneous/integrated pain:
- place preference conditioned by a non-rewarding analgesic
- testing the escape behavior paradigm
- Anxio-depressive disorders induced by chronic pain
- Unpredictable mild chronic stress
- Chronic variable stress
- Social defeat
- Open field
- Elevated-plus maze
- Light-dark test
- Marble burying test
- Social interaction test
- Forced swimming test
- Tail suspension test
- Sucrose preference test
- Splash test
- Nest test
- Novelty-suppressed feeding
- Place preference test
- Place aversion test
- Real-time seat preference test
- Tolerance, spontaneous withdrawal, precipitated withdrawal, scoring
- Active avoidance test
- Learned despair model
- Three-chamber social paradigm test
- Spontaneous alternations in Y-maze
- Object recognition
- Rotarod
- Actimetry
- Open field
- Traction reflex
- Postural asymmetry test
- Beam walking/horizontal bar
- SHIRPA test
- In collaboration with the Chronobiotron
- Complete system for recording and analyzing ultrasonic vocalizations of rats and mice (Ultravox XT)
- ANYmaze
- Stereotactic injections
- Cannula implantation
- Implementation of optical fibers
- Implementation of mini-osmotic pumps
- Cuff
- Chronic constriction injury
- Spared nerve injury
- Paw incision (post-operative pain)
- Intraperitoneal
- Subcutaneous
- Per os
- Intravenous
- Thoracic intrathecal
- Lumbar intrathecal
- Intracerebral
- Training in different tests, technical and surgical procedures
- Assistance in drafting animal experimentation authorizations
- Development of new models and/or behavioral tests
- Implementation of other existing tests
- Service provision
- Testing of therapeutic molecules
- Behavioral phenotyping
A Time-Dependent History of Mood Disorders in a murine model of neuropathic pain. Yalcin I, Bohren Y, Waltisperger E, Sage-Ciocca D, Yin JC, Freund-Mercier MJ, Barrot M. Biol Psychiatry, 2011, 70:946-953
The sciatic nerve cuffing model of neuropathic pain in mice. Yalcin I, Megat S, Barthas F, Waltisperger E, Kremer M, Salvat E, Barrot M. J Vis Exp, 2014, 89:e51608
Antidepressants and gabapentinoids in neuropathic pain: mechanistic insights. Kremer M, Salvat E, Muller A, Yalcin I, Barrot M. Neuroscience, 2016, 338:183-206.
A dual mechanism for duloxetine relief of neuropathic allodynia. Kremer M, Yalcin I, Goumon Y, Nexon L, Daniel D, Wurtz X, Megat S, Ceredig RA, Chavant V, Theroux JF, Lacaud A, Joganah LE, Lelievre V, Turecki G, Lutz PE, Ernst C, Gilsbach R, Salvat E, Barrot M. J Neurosci, 2018, 1004-18
How to study anxiety and depression in rodent models of chronic pain? Kremer M, Becker LJ, Barrot M, Yalcin I. Eur J Neurosci, 2021, 53:236-270