{"id":4126,"date":"2023-03-01T14:04:37","date_gmt":"2023-03-01T13:04:37","guid":{"rendered":"https:\/\/inci.neuro.unistra.fr\/?page_id=4126"},"modified":"2023-12-20T08:46:09","modified_gmt":"2023-12-20T07:46:09","slug":"qcr-quantitative-en-temps-reel","status":"publish","type":"page","link":"https:\/\/inci.neuro.unistra.fr\/?page_id=4126","title":{"rendered":"PCR quantitative en temps r\u00e9el"},"content":{"rendered":"\t\t
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PCR quantitative en temps r\u00e9el<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t

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P<\/b>rincipe et objectif<\/span><\/div>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t
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La PCR quantitative (PCR en temps r\u00e9el, QPCR)<\/em> utilise le principe de base de la PCR classique (amplification cyclique d\u2019un fragment d\u2019ADN bas\u00e9e sur une r\u00e9action enzymologique)<\/em> avec pour diff\u00e9rence une amplification mesur\u00e9e non pas en final mais tout au long de la r\u00e9action, et donc en temps r\u00e9el.<\/p>

A chaque cycle d\u2019amplification, la quantit\u00e9 d\u2019ADN est mesur\u00e9e gr\u00e2ce \u00e0 un marqueur fluorescent tel que le \u00ab syber green \u00bb dont l\u2019\u00e9mission est directement proportionnelle \u00e0 la quantit\u00e9 d\u2019amplicons produits. Ceci permet d\u2019obtenir une cin\u00e9tique de la r\u00e9action et donc la quantification de l\u2019ADN alors que la PCR classique ne donne que la mesure finale. Ainsi cette technique permet de comparer les diff\u00e9rences d\u2019expression des g\u00e8nes en fonctions des param\u00e8tres exp\u00e9rimentaux d\u00e9finis par le chercheur.<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t

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E<\/b>quipement<\/span><\/div>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t
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\"\"<\/a>L\u2019\u00e9quipe dispose d\u2019un thermocycler MyIQ de marque Biorad pour plaques 96 puits coupl\u00e9 \u00e0 une lampe UV et \u00e0 un spectrophotom\u00e8tre, le tout pilot\u00e9 par ordinateur gr\u00e2ce au logiciel BioRad IQ5.
thermocycler Mylq<\/p>

Cet \u00e9quipement se compl\u00e8te d\u2019un robot de pipetage \u00ab freedom evo \u00bb de marque TECAN qui permet de pr\u00e9parer les plaques PCR de 96 puits en un temps record (environ 7 minutes)<\/em> tout en \u00e9vitant les erreurs de pipetages ou de contaminations li\u00e9es \u00e0 l\u2019utilisateur. Ce robot de pipetage est particuli\u00e8rement int\u00e9ressant si le nombre de plaques \u00e0 effectuer est important. Ce robot est pilot\u00e9 via le logiciel Evoware de TECAN.<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t

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C<\/b>ontact<\/span><\/div>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t
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\"\"Le plateau technique de PCR quantitative est situ\u00e9 au 4e \u00e9tage l\u2019Institut (pi\u00e8ce 4312a)<\/em>.<\/p>

Il a pour mission de mettre \u00e0 disposition des chercheurs et des personnels de l\u2019INCI la technologie de QPCR.<\/p>

Support Scientifique<\/strong> : Vincent LELIEVRE<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t

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PCR quantitative en temps r\u00e9el La PCR quantitative (PCR en temps r\u00e9el, QPCR) utilise le principe de base de la PCR classique (amplification cyclique d\u2019un fragment d\u2019ADN bas\u00e9e sur une r\u00e9action enzymologique) avec pour diff\u00e9rence une amplification mesur\u00e9e non pas en final mais tout au long de la r\u00e9action, et donc en temps r\u00e9el. A […]<\/p>\n","protected":false},"author":1,"featured_media":0,"parent":10825,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"ocean_post_layout":"","ocean_both_sidebars_style":"","ocean_both_sidebars_content_width":0,"ocean_both_sidebars_sidebars_width":0,"ocean_sidebar":"0","ocean_second_sidebar":"0","ocean_disable_margins":"enable","ocean_add_body_class":"","ocean_shortcode_before_top_bar":"","ocean_shortcode_after_top_bar":"","ocean_shortcode_before_header":"","ocean_shortcode_after_header":"","ocean_has_shortcode":"","ocean_shortcode_after_title":"","ocean_shortcode_before_footer_widgets":"","ocean_shortcode_after_footer_widgets":"","ocean_shortcode_before_footer_bottom":"","ocean_shortcode_after_footer_bottom":"","ocean_display_top_bar":"default","ocean_display_header":"default","ocean_header_style":"","ocean_center_header_left_menu":"0","ocean_custom_header_template":"0","ocean_custom_logo":0,"ocean_custom_retina_logo":0,"ocean_custom_logo_max_width":0,"ocean_custom_logo_tablet_max_width":0,"ocean_custom_logo_mobile_max_width":0,"ocean_custom_logo_max_height":0,"ocean_custom_logo_tablet_max_height":0,"ocean_custom_logo_mobile_max_height":0,"ocean_header_custom_menu":"0","ocean_menu_typo_font_family":"0","ocean_menu_typo_font_subset":"","ocean_menu_typo_font_size":0,"ocean_menu_typo_font_size_tablet":0,"ocean_menu_typo_font_size_mobile":0,"ocean_menu_typo_font_size_unit":"px","ocean_menu_typo_font_weight":"","ocean_menu_typo_font_weight_tablet":"","ocean_menu_typo_font_weight_mobile":"","ocean_menu_typo_transform":"","ocean_menu_typo_transform_tablet":"","ocean_menu_typo_transform_mobile":"","ocean_menu_typo_line_height":0,"ocean_menu_typo_line_height_tablet":0,"ocean_menu_typo_line_height_mobile":0,"ocean_menu_typo_line_height_unit":"","ocean_menu_typo_spacing":0,"ocean_menu_typo_spacing_tablet":0,"ocean_menu_typo_spacing_mobile":0,"ocean_menu_typo_spacing_unit":"","ocean_menu_link_color":"","ocean_menu_link_color_hover":"","ocean_menu_link_color_active":"","ocean_menu_link_background":"","ocean_menu_link_hover_background":"","ocean_menu_link_active_background":"","ocean_menu_social_links_bg":"","ocean_menu_social_hover_links_bg":"","ocean_menu_social_links_color":"","ocean_menu_social_hover_links_color":"","ocean_disable_title":"default","ocean_disable_heading":"default","ocean_post_title":"","ocean_post_subheading":"","ocean_post_title_style":"","ocean_post_title_background_color":"","ocean_post_title_background":0,"ocean_post_title_bg_image_position":"","ocean_post_title_bg_image_attachment":"","ocean_post_title_bg_image_repeat":"","ocean_post_title_bg_image_size":"","ocean_post_title_height":0,"ocean_post_title_bg_overlay":0.5,"ocean_post_title_bg_overlay_color":"","ocean_disable_breadcrumbs":"default","ocean_breadcrumbs_color":"","ocean_breadcrumbs_separator_color":"","ocean_breadcrumbs_links_color":"","ocean_breadcrumbs_links_hover_color":"","ocean_display_footer_widgets":"default","ocean_display_footer_bottom":"default","ocean_custom_footer_template":"0","omw_enable_modal_window":"enable","footnotes":""},"class_list":["post-4126","page","type-page","status-publish","hentry","entry"],"yoast_head":"\nPCR quantitative en temps r\u00e9el - 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